EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Equipped with transmitted light (TL), green (Ex/Em: 460/541) and red (625/713) fluorescence channels, the MiniMax cytometer with SoftMax® Pro Software can be used to image and. Microplate Readers SpectraMax. Find regional support. Videos & Webinars SpectraMax i3x - Detection Module ChangeSpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. 2 only) SpectraMax M-series Microplate readers. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. Next,. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. The SpectraMax i3x Multi-Mode Microplate Reader has been validated for use with the ValitaTiter and ValitaTiter Plus assays. The resulting absorbance was measured at 490 nm, Spectra Max i3x (Molecular Devices, USA). Fourier transform infrared (FT-IR) spectra of cCQD were obtained on a. The chamber. Also refer to this Multimode Reader Comparison Chart. SpectraMax i3x plate reader (Molecular Device, contact supplier directly) Vacuum pump system (e. Biocompare is the leading resource for up-to-date product information, product reviews, and new technologies for life scientists. The SpectraMax ® i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. . The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. It allows the user to determine the total concentration of protein in a solution. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your intial investment. Review date: 19 Mar 2018 | SpectraMax i3x Multi-Mode Microplate Reader with MiniMax Imaging Cytometer The SpectraMax ® line of user-friendly microplate readers provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and moreSimilar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. i3x. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree TechnologySpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. Fluorescence Microplate Reader Comparison Chart. Page 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. Description 1. 22 μ m fi ltration unit (Sigma, cat. Videos & Webinars. ; India imports most of its Spectramax from United States, China and Austria and is the largest importer of Spectramax in the World. Transfected cells were imaged on the SpectraMax MiniMax 300 Imaging Cytometer. The NET-specificity of the detected signal was determined by incubation with DNase I (50 U/mL, Qiagen, Germany). SpectraMax i3x microplate reader and SoftMax Pro Software protocol. 0´ 104 8. Benefits • User-upgradeable application modules including cellular imaging • Sensitivity across spectrum with Spectral Fusion™ Illumination • Expanded dynamic range with cooled PMT • Control and analytics provided by SoftMax® Pro Software SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves. Bioz Stars score: 98/100, based on 1 PubMed citations. Detection cartridge modularity allows for easy addition of new read modes within minutes without. . Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader and Thermo Fisher Scientific’s Varioskan LUX reader also perform multiplexing. Learn how you can perform flash-based applications, such as dual luciferase and ATP assays, with high precision and accuracy using this cartridge. Meanwhile, the samples were detected with a fluorometric reader Spectra Max i3x (MD, America) at 488 nm excitation wavelength and 525 nm emission wavelength. As shown in the results above, the DELFIA cell proliferation assay combined with the SpectraMax i3 reader gives researchers a very sensitive test for measuring cell proliferation. Measured using the SpectraMax DuoLuc Reporter Assay Kit on the SpectraMax i3x microplate reader. The user guide also covers the features and specifications of the SpectraMax MiniMax 300. SpectraMax® i3x Multi-Mode Detection Platform, Molecular Devices. 1. When the user applies a set temperature of 37°C, the top is heated to 37. 12911151) 0. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel detection capabilities without the need for service. SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. • SpectraMax i3x Injector Cartridge with SmartInject™ Technology • Expand your research capabilities to include flash-based . With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user-configurable. Real-time measurement of extracellular oxygen depletion and acidification in cultured cells and microorganisms. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideSpectraMax® Microplate Readers and SoftMax® Pro Software. The following detection cartridge is required: • Fluorescence Polarization (FP-FLUO) Detection Cartridge (Molecular Devices cat. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. com. The SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. 0200-7029 Wavelength range (LUM): Visible to 650 nm Dead volume: < 10 µL with Reverse . The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. Synthesis of ZM-1NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. We would like to show you a description here but the site won’t allow us. Fluorescence intensity was measured using a Spectramax i3x plate reader (Molecular Devices) with excitation at 575 nm (bandwidth 9 nm) and emission at 608 nm (bandwidth 15 nm). Note: Additional readers with similar performance are listed. HeLa cells were plated at 15,000 cells per well in 100 µL of medium in a 96-well black, clear-bottom microplate. Data Analysis. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular. The plate was covered and incubated for one hour at room temperature. Structured Review. 3: The cuvette chamber. 0´ 105 dsD NA (p g/ wel ) R F USpectraMax Glo Steady-Luc 工作液都加 入到微孔板后，震荡摇匀并避光放置 10 min。使用 SpectraMax Glo Steady-Luc Reporter 内置的 SpectraMax Glo Steady- Luc Reporter 实验模板设定程序并通过 SpectraMax i3x 读板机检测发光。检测结 束后，模板会根据数据自动生成一条曲 线。SpectraMax MiniMax 300 细胞成像系统 为 SpectraMax i3x 多功能微孔板检测平台而打造！ • • • • 主要特点 系统全面兼容细胞成像 及微孔板检测技术 独特无标记分析技术无需 对细胞染色即可进行细胞 计数和贴壁度的计算 具有多种检测功能可对 细胞活力及毒性进行全. Benefits • User-upgradeable application modules including cellular imaging • Sensitivity across spectrum with Spectral Fusion™ Illumination • Expanded dynamic range with cooled PMT • Control and analytics provided by SoftMax® Pro Software SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an. Overview of SpectraMax MiniMax 300 Imaging Cytometer. 0001 0. 06, 0. Assay setup for a 384-well low-volume plate. Spectral Fusion Illumination is a powerful combination of Xenon flash lamp and LEDs that provides unmatched signal strengthThe SpectraMax i3x reader grows with you as your research areas expand. The corrected absorbance is within 5% of the value. However, no piece of equipment is immune to breaking down. VAL014, right table) assays on the SpectraMax i3x reader are shown below. 5 µL anti-cAMP-Cryptate. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Table 3 Optimal instrument settings for Valita Titer Plus assay Fluorescence Polarization measurement on Molecular Devices microplate readers. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. 2. D. 0´ 104 1. SpectraMax Quant dsDNA Assay Kits. Unpacking and setting up the instrument includes the following procedures: • Unpacking the instrument and saving the original packaging. The assay window spanned four orders of magnitude. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The possibility of user upgrading the system with an application cartridge and an imaging cytometer makes this device the most flexible microplate. The SpectraMax i3x Multi-mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with added functaionlity of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. Also refer to this Multimode Reader Comparison Chart. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. Spectramax I3x, supplied by Danaher Inc, used in various techniques. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Lower limits of detection for ATP determined using the ATPlite . Example: [Drawer]. Quantification of NETs was performed with the SpectraMax i3x microplate-reader (Molecular Devices, United Kingdom). It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. User-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. XPS spectra were obtained from PHI Versaprobe II. Native or oxidized AN (40 μM) and FN (0. Unlock your i3/i3x instrument after shipment. Danaher Inc spectramax i3x. SpectraMax® i3x Multi-Mode Detection Platform, Molecular Devices. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. After incubation at 37 °C and 5% CO 2 for 20 min, fluorescence images were taken using a Spectra Max i3x with MiniMax 300 Imaging Cytometer (Molecular Devices, San Jose, CA, USA) with the excitation and emission setup provided with SoftMax Pro 7. The dual auto injector module. 蛍光 発光 ELISA. The SpectraMax i3x’s user-upgradeable application modules will future-proof your research to include most assay capabilities of the SpectraMax iD3 and iD5 readers, as well as additional capabilities such as imaging. 08, 0. Bioz Stars score: 98/100, based on 1 PubMed citations. 6% for the on-board optics (monochromators). china@moldev. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. The difference in absorbance observed in the presence and absence of SOD was considered to be the amount of superoxide produced, and results. 2 (Molecular Devices, San Jose, CA). 11. Multimode Reader Comparison Chart. *On the SpectraMax i3x reader only. 5 decades, with sensitive detection of cells. no. Multimode Readers Comparison Chart. The fluorescence quantum yield (QY) of the Pdots was measured by a UV−NIR absolute PL QY. See SpectraMax® Microplate Reader User Manual Download Page. It allows you to explore cellular pathways and protein activation and expression in one system. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Spectramax I3x, supplied by Danaher Inc, used in various techniques. 2. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. The SPECTRAmax 340PC can mea-Learn how you can change the detection module of your SpectraMax i3x instrument in just a few seconds. Controls containing all reaction components with either DI water or a fluorescent standard in place of the fluorescent substrate were run alongside the experimental samples. Prepare your i3/i3x instrument for shipment. ) wide and 65 cm (25. CRITICAL STEP Avoid air bubbles; reverse pipetting the luciferase reagent into the plate is a good way to achieve this. Easily upgrade or switch between detection modes with user-exchangeable modules – just open the drawer and insert the module you want. 2. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. Align the two slots on the bottle holder with the two pegs on the rail. Wokingham, Berkshire RG41 5TS United Kingdom. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. i3. Figure 4. 313 reference gain check fail. Description 1. The SpectraMax® i Series Multi-Mode Mode Microplate Readers offer the ideal solution for any application or budget. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Bioz Stars score: 98/100, based on 1 PubMed citations. 4. Promega. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. The absorbance at 645 nm was subsequently determined using a Spectra Max i3x microplate reader (Molecular Devices). Patented user-exchangeable cartridge design expands the system's detection capability making it. The calculated LOQ values were 0. Carbonyl assay The plates for carbonyl detection were prepared as described. To detect physical stability, Pdots were treated with water and incubated with plants. GFP expression imaged on the cytometer. View online or download 1 Manuals for Molecular Devices SpectraMax i3x. Reporter Assay. The SpectraMax® i3x offers a wealth of applications; it can measure fluorescence, absorbance and luminescence, and its modular upgrades enable western blotting, imaging cytometry and injectors. In this case, the SpectraMax i3x reader works as a flash-lamp based microplate reader as described in the supporting application note “The AutoPMT™ feature for an extended dynamic range in the SpectraMax iD and M series Multi-Mode Readers”. 312 gain calibration failed. It allows you to explore cellular pathways and protein activation and expression in one system. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Spectranet customer portalWe would like to show you a description here but the site won’t allow us. We would like to show you a description here but the site won’t allow us. Molecular Devicesの全商品を見る. Introduction. Reaction catalyzed by the firefly luciferase. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email drugdiscoverytech@lifetech. Sunnyvale, CA, USA) with excitation at 475 nm. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. SoftMax Pro GxP software is a special release that includ es full user and permission control and audit trail generation in addition to all the datathe SpectraMax i3x Multi-Mode Microplate Reader with SpectraMax Injector Cartridge Introduction Reporter gene assays are used to study the expression of eukaryotic genes. 13. SpectraMax i3x 一套系统即可以帮助你通过科学手段解开细胞通路的奥秘，也可以检测并分析出 样品中蛋白质的活性和表达情况。SpectraMax i3x 检测系统可以根据未来实验需求，无需售后工 程师协助即可加入全新检测功能模块，真正做到一次投入终身受益。1) Select the correct plate and the wells that you want to measure. When used in combination with the SpectraMax Injector Cartridge, this system enables a wealth of flash type luminescence applications including reporter gene and other assays with exceptional sensitivity and dynamic range. 99（图一）。The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. Cet appareil ISM est confomre à la norme NMB-001 du Canada. For more information on the SpectraMax i3x Multi-Mode Microplate Reader System, please click here. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. After correcting for negative controls and quench standards, the soil enzyme activities were expressed in units of nmol h −1 g −1 soil (German et al. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. Register to download your free eBook today. HTRF-compatible SpectraMax Multi-Mode Readers. Accordingly, we calculated the Laurdan generalized polarization (GP) using the formula GP = (I 435 − I 490)/(I 435 +. The plate was shaken and incubated in the dark for 10 minutes. SpectraMax® iD3/iD5. We would like to show you a description here but the site won’t allow us. 2C). The MPO assay was conducted according to the. 1. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeSpectraMax MiniMax 300 イメージングサイトメーター は、研究室でのアップグレードが可能なSpectraMax i3xシステム専用のオプションで、ユーザーは複雑なイメージングシステムに投資することなく、細胞増殖を解析できます。SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. The SoftMax Pro Software quickly. Pick the best speed, sensitivity, and price for your needs. Art-science interactive light show/animation. The SoftMax Pro Software provides integrated instrument control. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. ATP LLD (pM) ATP LLD (moles) SpectraMax i3x, luminescence . 3: The cuvette chamber. *On the SpectraMax i3x reader only. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Using the SpectraMax i3x reader, the preconfigured SpectraMax Glo Steady-Luc Reporter assay protocol in SoftMax Pro Software was used to measure luminescence. Briefly, after exposed to ZnO/GO and Cd, cells were collected and washed with ice-cold PBS twice and then re-suspended in 200 μL binding buffer. Molecular Devices社の製品: 吸収度マルチモードマイクロプレートリーダー SpectraMax® i3x に関するすべての情報をご覧ください。. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Spectral Fusion Illumination is a powerful combination of Xenon flash lamp and LEDs that provides unmatched signal strength*On the SpectraMax i3x reader only. Find regional support. The SoftMax Pro Software provides integrated instrument control. SpectraMax iD5:Fluorescence was quantified using a microplate reader (SpectraMax i3x, Molecular Devices, CA, USA) with an excitation and emission wavelength being 365 nm and 450 nm, respectively (Jian et al. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,i3x Multi-Mode Microplate Reader. 0 | 9 reviews | Write your own review Measures Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgradesMolecular Devices SpectraMax i3x Multi-Mode Microplate Platform. The SpectraMax i3x Injector Cartridge with SmartInject Technology is a data sheet that describes the features and benefits of this innovative accessory for the SpectraMax i3x Multi-Mode Detection Platform. 3. Animal imaging assay was performed on a IVIS R Lumina Series III small animal optical system. After incubation at 37 °C and 5% CO 2 for 20 min, fluorescence images were taken using Spectra Max i3x with MiniMax 300 Imaging Cytometer (Molecular Devices, San Jose, CA) using the setup of excitation and emission provided in the SoftMax Pro 7. Introduction. Prepare your iD series instrument for shipment. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. 313 reference gain check fail. SpectraMax i3x Multi-Mode Microplate Reader; SpectraMax MiniMax 300 Imaging Cytometer *Here, the NucView 488 with DMSO formulation was used. Donor emission at 616 nm is used as an internal reference, while acceptor emission at 665 nm. Learn how you can perform flash-based applications, such as dual luciferase and ATP assays, with high precision and accuracy using this cartridge. • Removing the transport locks from the PMT shutters and drawers. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes This assay workflow is easily performed using the SpectraMax i3x Multi-Mode Microplate Reader with SpectraMax Injector Cartridge, which is fully DL Ready validated. 001 0. The soil suspension (600 µL) and the substrate (L-3,4-dihydroxyphenylalanine, DOPA, 150 uL, 25 mmol L -1 ), were mixed and then added to. Besides, it’s possible to examine each page of the guide singly by using the scroll bar. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. X-ray powder diffractometer (XRD) with Cu- Ka radiation (Bruker AXS D8) was used to identify the crystalline phase of as synthesized C-AuNPs. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. See SpectraMax® Microplate Reader User Manual Download Page. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™certified. 性能特点MD SpectraMax i3X 多功能荧光检测仪为采用光谱融合照明设计、测试功能模块化卡盒设计，具有细胞成像分析单元。. 303 unable to cal dark current. The SpectraMax Injector Cartridge enables precise reagent delivery and adjustment of delay and integration times that are required to obtain optimal sensitivity for this assay. techsupport. Examples: fiPress carefully to engage,fl fiDo not press down. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. SpectraMax i3x Multi-Mode Detection Platform User Guide. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. We would like to show you a description here but the site won’t allow us. Besides, it’s possible to examine each page of the guide singly by using the scroll bar. ZERO BIAS - scores, article reviews, protocol conditions and more. It allows you to explore cellular pathways and protein activation and expression in one system. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. The ScanLater Western Blot Detection Cartridge is a user-installable option for the SpectraMax i3, i3x and Paradigm Multi-Mode Readers. After correcting for negative controls and quench standards, the soil enzyme activities were expressed in units of nmol h −1 g −1 soil (German et al. Microplate assays were performed on a multimode microplate detector (Molecular Devices Spectra Max i3x). Superior flexibility The SpectraMax i3 Multi-Mode Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. These robust readers have been placed in labs from Antarctica to the. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. The SpectraMax i3 Multi-Mode Detection Platform is a versatile instrument that can perform various assays and measurements on microplates and cuvettes. The SpectraMax ® i3x reader provides the. Itemploysa luciferaselabel(where“label”generallymeansfusedbytheuseofexpressionvectors)forthe oneproteinandaGreenFluorescentProtein(GFP)variantlabelfortheother. The SpectraMax® i3 Platform from Molecular Devices® is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. ) deep, preferably with access on all sides. 1 mg/mL density of resazurin, protected of the light, incubated for 1 h at room temperature. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. Contact Local Team. The SpectraMax i3x multi-functional detection platform launched by Molecular Devices can meet the multi-functional detection system with unlimited possible applications as your future needs change. o For example: Ex: 485/Em 525/Cutoff 515 4. Analysis of HTRF assays involves Cisbio’s patented ratiometric reduction method based on the two emission wavelengths detected. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. 在SpectraMax i3x和SpectraMax iD5微孔板读板机上使用 SoftMax Pro软件绘制标准曲线，并进行4参数曲线拟合(图 4)，结果见表2。使用EC50评估该测定方法的适用性，EC50值 应低于150 nM。对于两个读板机，EC 50值均低于85 nM，处 于可接受范围内。Cisbio将可接受的信噪比定义为≥. Analysis. SpectraMax i3x读板机针对96孔板和384孔板进行检测时，最低检测下限可至10细胞每孔。 此种检测方法的灵敏度远远高于标准比色法和绝大多数荧光法。 CellTiter-Glo试剂检测线性范围覆盖所有细胞浓度区间，动态学检测范围大于3个数量级且r2 > 0. Spectranet customer portalThe Relative Luminescence Units (RLU) were measured using the SpectraMax i3x Luminescence Glow cartridge (Lum 384) (0200-7015POS, Molecular Devices) and normalized to the cell count or area, which were determined using the Mini MaxTM 300 Imaging Cytometer (5024062, Molecular Devices). The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Explore a wealth of applications in one future-ready system that measures spectral-based absorbance, fluorescence, and luminescence. Learn how you can change the detection module of your SpectraMax i3x instrument in just a few seconds. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the. Controls containing all reaction components with either DI . Recommendations for SpectraMax i3/i3x plate readers The Molecular Devices SpectraMax i3/i3x allows use of the pH-Xtra Glycolysis Assay with time-resolved fluorescence (TRF) detection. Published Dec 16, 2020 | Updated Dec 16, 2020. infoboxeu@moldev. Molecular Devices Software. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. SpectraMax i3x, onboard luminescence 121. The possibility of user upgrading the system with an application cartridge and an imaging cytometer makes this device the most flexible microplate. Dilution series of HeLa cells transfected with firefly (red plot) and Renilla (blue plot) luciferases. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. Check software compatibility with Windows 10 and locate your software installer. Video: SpectraMax i3x Overview; Brochure: SpectraMax i3x Multi-Mode Detection PlatformNORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Detection cartridge modularity allows for easy addition of new read modes within minutes without. Prepare your iD series instrument for shipment. Kastelic,2 and Jian Gao 1 1Department of Clinical Veterinary Medicine, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China. User-upgradeable applications The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. It can be upgraded with modules such as imaging cytometer, western blot imager, and injectors to expand its capabilities. Fluorescent Polarization: Solid black plates, as above. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. Our patent-pending StainFree Cell Detection Algorithm enables cell counting and confluency measurements. Leveraging this novel technology, researchers can: Add western blot detection to their microplate reader within 2 minutes. SpectraMax i3x Multi-Mode Detection Platform. The SpectraMax® i3 Platform's base system features an integrated optical system enabling top and bottom reads for 6-, 384- well microplates and launches with three broad detection modes: luminescence, absorbance, and fluorescence. GFP expression was higher at 21 hours post transfection than at 16 hours (16-hour data not shown). Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. 3, were mixed in a final volume of 100 μL in 10 mM sodium phosphate buffer, pH 7. com The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree Technology*On the SpectraMax i3x reader only. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Examples: “Press carefully to engage,” “ Do not. The SPECTRAmax 340PC can mea- Learn how you can change the detection module of your SpectraMax i3x instrument in just a few seconds. edu) to be added as a trained user in iLab. 在SpectraMax i3x和SpectraMax iD5微孔板读板机上使用 SoftMax Pro软件绘制标准曲线，并进行4参数曲线拟合(图 4)，结果见表2。使用EC50评估该测定方法的适用性，EC50值 应低于150 nM。对于两个读板机，EC 50值均低于85 nM，处 于可接受范围内。Cisbio将可接受的信噪比定义为≥. Table 1. For SpectraMax iD5, i3x, and Mini readers, the read height setting should be optimized for the microplate used. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. 200-800 nm using Spectra Max i3X microplate reader. CHO M1WT3 cells are Chinese hamster ovary cells stably transfected with the M1 muscarinic G q -coupled receptor. Hands-on fun and learning that empowers exploring, inventing and sharing. Fluorescence was measured using a microplate fluorometer (SpectraMax i3x, Molecular Devices, Beckman Coulter, CA, USA) with 365 nm excitation and 450 nm emission filters. ; The top 3 importers of Spectramax are India with 365. Fluorescence Microplate Reader Comparison Chart. Flow cytometry was used to measure ROS concentrations, based on fluorescence intensity (FL-1, 530 nm) of 10,000 cells. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,Danaher Inc spectramax i3x Spectramax I3x, supplied by Danaher Inc, used in various techniques. Absorbance: For wavelengths in the visible range, 340 nm and above, clear polystyrene plates are recommended. User-upgradeable detection modules allow you to expand the capabilities of your microp. #0200-7009) The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. flValitaTiter (i3x i3) ValitaTiter (iD5) ValitaTiter (M5 M5e Flex3) ValitaTiter (Paradigm) ValitaTiter (FilterMax F5) Assay-Specific. Budget-friendly multi-mode microplate reader . Data Analysis. 8 Antibodies and. Spectranet customer portal The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree Technology The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. 315 can't find zero order. Home > Search Results > Danaher Inc > spectramax i3x. 1step assay with SpectraMax microplate readers. Get a front row view of your cells. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. This way you’ll save time on finding the necessary info. User-installable and removable detection cartridges allow the detector to be configured for specific applications and easily. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. . 3. 1、卡盒式模块和细胞成像模块的构架检测功能（如FP、TRF、HTRF、AlphaScreen、ScanLater Western Blot等）卡盒模块化设计，仅通过插入卡盒就能可. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. The SpectraMax i3x reader provides theWith these results, as well as CV values below 6% for all standards, both the SpectraMax i3x and the SpectraMax iD5 readers demonstrate high sensitivity and a wide dynamic range for the IP-One assay. About the reader setup recommendations The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Bound crystal violet was dissolved with 10% acetic acid and the optical density (OD) at 570 nm was determined using a Spectra Max i3X spectrophotometer (Molecular Devices). Note: Additional readers with similar performance are listed. Open the catalog to page 1. 800. ATP (“flash” luminescence using . SpectraMax i3x and Paradigm readers require the FP-FLUO. 635. Prepare your i3/i3x instrument for shipment. Detection of pro-inflammatory factors. Published Dec 16, 2020 | Updated Dec 16, 2020. Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries. The CellTiter-Glo assay from Promega uses luciferase. Accessories for SpectraMax® i3x Multi-Mode Detection Platform, Molecular Devices. It allows you to explore cellular pathways and protein activation and expression in one system. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Molecular Devices社の製品: 吸収度マルチモードマイクロプレートリーダー SpectraMax® i3x に関するすべての情報をご覧ください。. Contact our support team with your questions regarding Molecular Devices products and applications. 2 only) SpectraMax M-series Microplate readers. ; Page 2 Molecular Devices equipment, software, reagents, and consumables. Preconfigured protocols and custom assay workflows simplify microplate data acquisition and analysis. Microplate and read height should be optimized for each new lot of microplates or assay volume used (use the Show Pre-Read Optimization options in the instrument settings in SoftMax Pro Software). The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. Bioz Stars score: 98/100, based on 1 PubMed citations. Supplier: Molecular Devices. com. Be Future Ready with the SpectraMax i3x Multi-Mode Detection Platform. Amplitude : 1. Learn more about its features, specifications, and applications in this user guide. 99. Molecular Devices are known for both the reliability and the wide uses of their products. Features. 306 plate air cal. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Promega. Multimode Readers Comparison Chart. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors.